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KMID : 0370220080520010001
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Yakhak Hoeji
2008 Volume.52 No. 1 p.1 ~ p.6
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Apoptosis Induction of HL-60 Leukemia Cells by Extract of Crinum asiaticum
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Hyoun Jae-Hee
Kim Elvira
Kang Jung-Il
Kim Sang-Chul
Yoo Eun-Sook
Kang Hee-Kyoung
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Abstract
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The present study investigated the antiproliferative effects of Crnum asiaticum var. japonicum against HL-60 human leukemia cells. The 80% MeOH extract or several solvent fractions from the C. asiaticum inhibited the growth of HL-60 cells, whereas the growth of HEL-299 cells, human embryonic lung fibroblast, was scarcely inhibited. When the HL-60 cells were treated with the CHCL₃fraction, the BuOH fraction, the EtOAc fraction and the H₂O fraction, DNA ladder, chromatin condensation and increase of sub-G1 hypodiploid cells were observed. Furthermore, the CHCL₃ fraction and the BuOH fraction reduced Bc1-2 mRNA level, whereas Bax mRNA level was increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60 cell might be mediated through the induction of apoptosis via the down-regulation of Bc1-2. Taken together, components of C. asiaticum might have a therapeutic potential for the treatment of human leukemia.
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KEYWORD
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Crinum asiaticum, HL-60 cell, Apoptosis
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